By U Wahnschaffe; United Nations Environment Programme.; International Labour Organisation.; World Health Organization.; Inter-Organization Programme for the Sound Management of Chemicals.; et al; All authors
ISBN-10: 9241572337
ISBN-13: 9789241572330
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Extra resources for Transgenic animal mutagenicity assays
Sample text
2 Generation of transgenic animals with shuttle vectors: the transfer method Big Blue® animals and the Muta™Mouse have been produced by pronucleus microinjection (Fig. 1), a technique that is currently the most successful and most widely used method of producing transgenic animals. The method allows an early integration of the transgene into the host DNA, which is important to ensure that transgenic DNA is apparent in all cells of the host. , 1980; Gordon & Ruddle, 1983). Male and female pronuclei are microscopically visible several hours following 10 Construction of Transgenic Animals the entry of the sperm into the oocyte.
1991a). , 1998). For the construction of Muta™Mouse, fertilized eggs of CD-2 F1 (BALB/c × DBA/2) were microinjected with 150 copies of the monomeric Ȝgt10LacZ vector. Four mice with different copy numbers were selected to be bred into strains. , 1989). 4 Target or reporter genes — the lac operon Several target genes for mutations are currently used in genotoxicity testing in mammalian models. From these, the bacterial lacI 12 Construction of Transgenic Animals and lacZ genes have been studied in most detail.
An international workshop of experts (IWGT) convened to discuss transgenic mutation assays in Washington, DC, USA, in 1999. , 2003). Other systems, such as the ĭX174 transgenic model, are also briefly described here. 1 ȜcII assay (Big Blue® and Muta™Mouse) The cII gene (294 bp) is a Ȝ phage gene (see Fig. 4 and Fig. 7). The level of cII protein plays a central role in the lytic-lysogenic commitment made by Ȝ phage upon infection of E. coli. The cII protein activates the transcription of the genes of the cI repressor protein and of the Ȝ phage integrase protein, which are required for the establishment of lysogeny.
Transgenic animal mutagenicity assays by U Wahnschaffe; United Nations Environment Programme.; International Labour Organisation.; World Health Organization.; Inter-Organization Programme for the Sound Management of Chemicals.; et al; All authors
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