By Robert E. Moore (auth.), William B. Coleman, Gregory J. Tsongalis (eds.)
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Extra info for Molecular Diagnostics: For the Clinical Laboratorian
Coleman 30 Normal Human Chromosome Large Deletion of the Short Arm Amplification Translocation of a of DNA Sequences Chromosome Segment on the Short Arm to the Long Arm Fig. 6. Forms of gross chromosomal aberrations. Three examples of chromosomal aberrations are demonstrated using a standard G-band ideogram of human chromosome 11. The asterisks denote the chromosomal band that has expanded owing to DNA amplification. The stippled area highlights a region from another chromosome that has been translocated to the long arm of the starting chromosome, displacing the normal qter region without altering the overall size of the chromosome (note the differences in G-banding in this region).
However, it is not clear whether this effect is related to a direct influence of poly(A) structures on the initiation reaction or the result of some indirect cause. 26 Coleman Some other forms of posttranscriptional RNA processing occur with respect to a small subset of eukaryotic mRNAs. The process of RNA editing involves a posttranscriptional alteration of the informational content ofa specific mRNA (23). The editing of RNA is revealed when the linear sequence of the mRNA molecule differs from the coding sequence carried in the DNA.
The basis for this method is that proteinase K, an enzyme that degrades proteins, is highly active in this mixture, whereas DNA-degrading nuc1eases are greatly inhibited. This incubation is followed by a series of organic extractions generally employing mixtures of phenol, chloroform, and isoamyl alcohol. The organics are mixed with the DNAcontaining sample, creating an emulsion, to dissolve hydrophobic contaminants and further denature and remove proteins. Since the organics are not cosoluble with the aqueous sample, they separate from each other into two phases (this is often facilitated by centrifugation).
Molecular Diagnostics: For the Clinical Laboratorian by Robert E. Moore (auth.), William B. Coleman, Gregory J. Tsongalis (eds.)