By Baldwin H. Tom (auth.), Baldwin H. Tom, James P. Allison (eds.)
The skill to "immortalize" immunologically-useful cells through hybridization with a special melanoma phone has revolutionized serological experiences and has published new strength purposes in all fields of organic sciences. This quantity offers the reports from a hugely winning nationwide symposium on Hybridomas and mobile Immortality held November 1981 in Houston, Texas. the person chapters express the range of themes mentioned through the assembly. those comprise emphasis at the beginning of antibody variety, Band T lymphocyte differentiation, functions of monoclonal antibodies in reviews of histocompatibility, tumor, and viral antigens, plus using somatic cellphone hybridizations for learning T cellphone items. 3 papers specialise in the rising methodologies of in vitro basic immunizations for either humoral and cell-mediated immunities, proper for coupling with hybridoma expertise. there's a important mixture of normal (methods) and particular (applications) chapters. a special element of the e-book is the presentation of either contemporary examine findings with concise descriptions of the state-of-the-art methodologies. it really is expected that this paintings may be of curiosity to a large viewers of practioners in biomedical learn. confidently, the knowledge contained will foster new and imagi local rules in hybridoma purposes. Baldwin H. Tom, Ph.D. James P. Allison, Ph.D. vii CONTENTS half L advent TO HYBRIDOMAS 1 Somatic telephone Hybrids and Hybridomas Baldwin H. Tom three 1. Somatic cellphone Hybrids eight Hybridomas. • • • • • 2.
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The facility to "immortalize" immunologically-useful cells via hybridization with a special melanoma mobile has revolutionized serological reviews and has published new capability functions in all fields of organic sciences. This quantity provides the experiences from a hugely winning nationwide symposium on Hybridomas and mobile Immortality held November 1981 in Houston, Texas.
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Extra resources for Hybridomas and Cellular Immortality
4. 9. In all instances it was reported that these monoclonals detected a molecule expressed on all normal T cells, that was not present on normal B cells or monocytes. , 1980). Since most of these tumors were clearly of the B cell lineage, it was initially viewed that these monoclonals were useful as pan T reagents when used with normal cell populations, but that in some lymphoid malignancies, specifically CLL, aberrant expression of the Leu-l antigen occurred. , 1981a). , 1981). Further studies have questioned whether expression of Ly1 on murine B cell tumors is in fact reflective of a similar expression by at least a subset of normal mouse B cells.
1980. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes. Nature 286:676683. , Weigert, M. , 1981. Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes. Nature 290:562-565. , Tiemeier, D. , 1978a. Multiple related immunoglobulin variableregion genes identified by cloning and sequence analysis. Proc. Natl. Acad. Sci. USA 75:3881-3885. , Norman, B. , 1978b. Antibody diversity. Science 202:11-17.
All samples were treated with the indicated first stage reagents (anti-Leu-2a, anti-Leu-3a, MOPC-21 control myeloma protein and Fab fragment of anti-Leu-3a), followed by FITC goat anti-mouse immunoglobulin. All three intact monoclonal antibodies are of IgG isotype. Note that both intact and Fab fragment of anti-Leu-3a showed marked reactivity with the U937 cell line, whereas there was no staining with anti-Leu-2a or a control MOPC-21 myeloma protein. and malignant histiocytosis in skin and spleen.
Hybridomas and Cellular Immortality by Baldwin H. Tom (auth.), Baldwin H. Tom, James P. Allison (eds.)