By Jacob M. Jaworski
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Physical factors in benthic ecology: effects of changing sand particle size on beach fauna. Marine Ecology, 131, 205-217. McLachlan, A; Van der Horst, G. (1979). Growth and reproduction of two mollusks from an exposed sandy beach. South African Journal of Zoology, 14, 194-201. McLachlan, A; Jaramillo, E; Donn, T; Wessels, F. (1993). Sandy beach macrofauna communities and their control by the physical environment: a geographical comparison. Journal of Coastal Research, 15, 27-38. Mistri, M; Rossi, R.
35 Ballygunge Circular Road, Kolkata – 700019, India ABSTRACT Sundarbans biosphere reserve is a unique ecosystem and characteristic of diverse types of habitats ranging from mudflat to sandy beaches. Nutritionally, economically and ecologically important estuarine edible crab, Scylla serrata (Arthropoda: Crustacea: Decapoda) occupy diverse habitats and their survival is dependent on multidimensional immune responses mostly offered by hemocytes – the chief immunoeffector cells of hemolymph. The circulating hemocyte number is a stress indicator and hemocyte counts may be a valuable tool in monitoring the health status in biounsafe environment in S.
Smear cells were subjected to treat with methanol for 2-3 min for fixation. Unstained fix cells were examined under phase contrast microscope (Axiovert, Zeiss, Germany) prior to stain. The number of hyalinocytes, semigranulocytes and granulocytes (differential hemocyte count, DHC) were estimated using bright field after fixing and staining hemocytes with Wright stain (SRL, India) throughout the study and taken digital photograph by camera-attached microscope (Axiostar Zeiss, Germany). Number of each cell type was expressed in percentage (Saha and Ray, 2006).
Animal Science and Issues by Jacob M. Jaworski