By T.K. Ghose ,A. Fiechter, N.Blakebrough
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Extra resources for Advances in Biochemical Engineering, Volume 003
13 shows the metabolites formed under the conditions of blocked chlorination. The final product is TC. Fig. 14 shows the formation @ NH2 2 x Me OH-C6 C[ CONH2 Me NMe2 H "y"U'~'~'CONH2 OH 0 OH OH C,I Me OH NMe2 "U "r" " ~ " ~ "CONH2 OH 0 OH OH methylchlor t e t r o m i d - blue el el Me OH 0 ~ c O ~ N H 2 OH 0 OH 0 chlorletrarnid - green Fig. 14. , 197l) 51 Genetic Problems of the Biosynthesis of Tetracycline Antibiotics ® NH2 2 x Me 0H- C6 M=e NIM%H Me OH N,MeaoH CONH2 OH O OH 0 OH OH CONH2 OH OH methyltetramid - blue BI Me OH _ _0 CONH 2 tetromid - ~reen Fig.
The structure of these compounds and their physico-chemical properties have not been described. , 1961; Boronin and Mindlin, 1970). The nonpigmented "white'" mutants of group 6 were cosynthetically active only in combination with the "black" mutants of group 3. The "white" mutants were found to produce the so-called X-factor. 1-~. 0 1--20 Antibiotic activity (gg/ml) + + + + + + blue yellow violet light blue + + -- -- --- + + + -- --- ....... + . . . . . . violet + . . . . . . . . .
The above facts also indicate that one cannot draw a precise boundary between primary and secondary metabolism of natural compound producers. It is likely that a single general common metabolic pattern exists and that various regulatory mechanisms determine the flow of intermediates and the intensity of the individual competing metabolic pathways. The production activity is then the result of interaction of the various control levels. From this point of view then the terms secondary metabolism and secondary metabolite seem to be redundant.
Advances in Biochemical Engineering, Volume 003 by T.K. Ghose ,A. Fiechter, N.Blakebrough