By David A. Fitzpatrick, Geraldine Butler (auth.), Ruth Ashbee, Elaine M. Bignell (eds.)
Mycological reviews of yeasts are getting into a brand new section, with the sequencing of a number of fungal genomes informing our knowing in their skill to reason ailment and engage with the host. even as, the continued use of conventional equipment in lots of scientific mycology laboratories keeps to supply details for the analysis and therapy of sufferers. This quantity experiences quite a few points of pathogenic yeasts and what's recognized approximately their molecular and mobile biology and virulence, as well as taking a look at scientific and laboratory findings. As each one bankruptcy is written by means of a number one specialist within the box, this booklet summarizes in a single quantity a lot of the most recent examine on numerous pathogenic yeasts, together with Candida, Cryptococcus, Malassezia and yeasts of rising value. the significance of laboratory prognosis, antifungal susceptibility checking out, antifungal resistance and yeast ailments in animals are reviewed.
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Additional info for Pathogenic yeasts
2007), Pasqualotto et al. (2007), Sarvikivi et al. (2008), Schelenz and Gransden (2003), Shivaprakasha et al. (2007), St-Germain et al. (2008), Swinne et al. (2009) Admission to intensive care Celebi et al. (2008), Cheng et al. (2005b), Franca et al. (2008), unit (ICU) Odds et al. (2007), Sarvikivi et al. (2008), Schelenz and Gransden (2003), Shivaprakasha et al. (2007), St-Germain et al. (2008), Swinne et al. (2009) Surgery (including organ Bassetti et al. (2006), Chow et al. (2008), Colombo et al.
Widespread use of cytotoxic therapies to treat cancer and the use of immunosuppressive drugs in organ transplantation have greatly increased the number of neutropenic patients in intensive care units. Risk factors for infection include cancer, extremes of age, prior colonisation and the presence of intravenous catheters (Pittet et al. 1994; Wenzel 1995). Recently, Martin et al. (2003) analysed the rate of sepsis in hospitals in the USA from 1979 to 2000 and found that the rate of sepsis due to fungal organisms increased by 207%.
The nature of DNA sequence analysis means that the data are reproducible and unambiguous. MLST is a highly discriminatory method that relies on the analysis of nucleotide sequence polymorphisms within the sequences of PCR-generated fragments (400–500 bp) of 6–8 housekeeping genes (loci) (Odds and Jacobsen, 2008). An outline of the procedure is shown in Fig. 1. In haploid organisms, the sequences obtained at each locus are assigned as discreet alleles, and for each isolate the combination of alleles define an allelic profile, or sequence type (ST).
Pathogenic yeasts by David A. Fitzpatrick, Geraldine Butler (auth.), Ruth Ashbee, Elaine M. Bignell (eds.)