By Galileo Galilei
Indici dei XX volumi, 17
Indice dei facsimili, 47
Indice dei nomi e delle cose notabili, 55
Indice biografico (persone collegate a Galileo Galilei), 359
Supplemento al Carteggio, 567
Supplemento ai Documenti, 585
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Extra resources for Le Opere di Galileo Galilei, Edizione Nazionale
Example text
This should provide a more reliable indication of infection than the present methods which rely primarily on visual iridescence of infected insects. , 1993). 9 kDa, is slightly higher than that predicted from SDS-PAGE analyses (49 kDa). In an earlier study, Moore and Kelly (1980) showed that the major capsid protein of three other insect IVs had an N-terminal proline and, therefore, it has been suggested that the capsid of IV22 may be post-translationally processed to give the apparent molecular mass identified on gels (Cameron, 1990).
EPV, isolated by treatment with the non-ionic detergent NP-40, have been shown to contain only one major protein VP59 (Bilimoria and Arif, 1980). Four enzymatic activities have been associated with the virion particles of AmEPV: a nucleotide phosphohydrolase (Pogo et af. , 1971), and a DNA-dependent RNA polymerase (McCarthy et a f . , 1974). An endogenous alkaline proteolytic activity has also been reported to be associated with occlusion bodies isolated from insect larvae. Spheroidin was degraded from a 102 kDa protein to a 52 kDa polypeptide and eventually into smaller polypeptides when the spheroids were dissolved in alkali (Bilimoria and Arif, 1979).
Recircularization of linear BacPAK6 DNA produces a crippled virus which is unable to produce infectious virus in insect cells. If the same DNA was co-transfected with a plasmid transfer vector, however, virus yields were enhanced considerably with nearly 100% recovery of recombinant virus. Very few parental (BacPAK6) viruses are evident in the first round of plaque purification, enabling rapid isolation of recombinant viruses. The co-transfection of linear BacPAK6 DNA with the transfer vector serves to repair the partial deletion in ORF 1629 and permit the production of infectious virus.
Le Opere di Galileo Galilei, Edizione Nazionale by Galileo Galilei
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