Download e-book for kindle: Le Opere di Galileo Galilei, Edizione Nazionale by Galileo Galilei

By Galileo Galilei

Indici dei XX volumi, 17
Indice dei facsimili, 47
Indice dei nomi e delle cose notabili, 55
Indice biografico (persone collegate a Galileo Galilei), 359
Supplemento al Carteggio, 567
Supplemento ai Documenti, 585

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Extra resources for Le Opere di Galileo Galilei, Edizione Nazionale

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This should provide a more reliable indication of infection than the present methods which rely primarily on visual iridescence of infected insects. , 1993). 9 kDa, is slightly higher than that predicted from SDS-PAGE analyses (49 kDa). In an earlier study, Moore and Kelly (1980) showed that the major capsid protein of three other insect IVs had an N-terminal proline and, therefore, it has been suggested that the capsid of IV22 may be post-translationally processed to give the apparent molecular mass identified on gels (Cameron, 1990).

EPV, isolated by treatment with the non-ionic detergent NP-40, have been shown to contain only one major protein VP59 (Bilimoria and Arif, 1980). Four enzymatic activities have been associated with the virion particles of AmEPV: a nucleotide phosphohydrolase (Pogo et af. , 1971), and a DNA-dependent RNA polymerase (McCarthy et a f . , 1974). An endogenous alkaline proteolytic activity has also been reported to be associated with occlusion bodies isolated from insect larvae. Spheroidin was degraded from a 102 kDa protein to a 52 kDa polypeptide and eventually into smaller polypeptides when the spheroids were dissolved in alkali (Bilimoria and Arif, 1979).

Recircularization of linear BacPAK6 DNA produces a crippled virus which is unable to produce infectious virus in insect cells. If the same DNA was co-transfected with a plasmid transfer vector, however, virus yields were enhanced considerably with nearly 100% recovery of recombinant virus. Very few parental (BacPAK6) viruses are evident in the first round of plaque purification, enabling rapid isolation of recombinant viruses. The co-transfection of linear BacPAK6 DNA with the transfer vector serves to repair the partial deletion in ORF 1629 and permit the production of infectious virus.

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Le Opere di Galileo Galilei, Edizione Nazionale by Galileo Galilei

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