ISBN-10: 0470514345
ISBN-13: 9780470514344
ISBN-10: 047193447X
ISBN-13: 9780471934479
A complete evaluate of present pondering at the biosynthesis, functionality and evolution of secondary metabolites in animals, crops and microorganisms. Examines the normal context of secondary metabolites as common items having no seen half to play within the generating organism's existence cycle. Covers matters on the topic of genetic and antibiotic functions.
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Extra info for Ciba Foundation Symposium 171 - Secondary Metabolites: their Function and Evolution
Sample text
We have additional experiments in progress. Turner: To go back to my earlier point about the type of intron you studied, how widespread are group I introns? Mitochondria don’t have introns at all in higher eukaryotes, for example. Davies: No, but I think the experiments should be done in bacteria or fungi to begin with, and nobody has yet looked for splicing inhibition in higher eukaryotes. Cavalier-Smith: Have you even tried it with Tetrahymena group I introns? It might not even be true for all group I introns.
The 2-deoxystreptamine compounds presumably act at a different site within the intron, because inhibition is not reversed by the addition of guanosine. Demain: How about 2-deoxystreptamine itself? Davies: It doesn’t work. Orgel: I noticed a considerable jump between the main part of your paper, the experiments, and the conclusions. The talk referred to the action of a small group of molecules already known to interact with polynucleotides, but your conclusions were very broad and global. Perhaps you should restrict the conclusions to those antibiotics which are known to interact with RNA in this way?
Guanine is required as the promoter of the self-splicing of group I introns, and competitive inhibition of guanine binding by guanosine analogues or a molecule containing a guanidino group, such as arginine, leads to a reduction in the formation of the products of the first step of splicing (Cech 1990). Kinetic studies of inhibition by streptomycin showed that it too was a competitive inhibitor of guanine’s binding to its intron site (von Ahsen & Schroeder 1991). In contrast, a number of aminoglycosides of the 2-deoxystreptamine (DOS) class (Fig.
Ciba Foundation Symposium 171 - Secondary Metabolites: their Function and Evolution
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