By N. E. Fusenig (auth.), N. E. Fusenig, H. Graf (eds.)
Cell biology has made an considerable influence at the evaluate of physiological and pathophysiological approaches resulting in a extra precise realizing of the signaling mechanisms wherein cells speak in vivo and in vitro and alter adaptively. by utilizing cellphone tradition versions as well as animal experiments we're now in a position to larger outline the final and the selective power of gear. This ebook is designed to offer info at the merits and boundaries and on new features and the that means of mobile tradition versions in pharmaceutical research.
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Extra resources for Cell Culture in Pharmaceutical Research
1989 Braun et al. 1989, 1990 Montarras et al. 1991 Rohwedel et al. 1994 tlatlOn during mammalian development (reviewed by Olson 1990; Buckingham 1992). The expression of these myogenic determination genes is developmentally controlled during mouse embryogenesis (Sassoon et al. 1989; Bober et al. 1991; Hinterberger et al. 1991, Ott et al. 1991). Several muscle cell lines have been established from rat (Yaffe 1968) and mouse (Schubert et al. 1974; Yaffe and Saxe11977; Davis et ai. 1987; Mulle et al.
While cardiomyocytes of early differentiation stage (7 + 2 to 7 + 4 days) revealed only pacemakerlike action potentials, three major types of action potentials were found in cardiomyocytes at the terminal differentiation stage (7 + 9 to 7 + 12 days). Cells of atrial and ventricular phenotypes were characterized by a stable resting potential of about -75 mV and by action potentials of high amplitude and upstroke velocity. Similarly as described for adult myocardium (Irisawa 1989; Trautwein and Hesche1er 1990), atrial action potentials differed from ventricular action potentials by showing a less pronounced plateau and by hyperpolarizing under muscarinic cholinoceptor agonists (Fig.
ES cells (BLC 6) cultivated on feeder layer Time schedule (days) o . Preparation of cell suspension and cultivation of 800 cells/20 III medium in hanging drops IIV Gv V I • Formation of embryo-like aggregates (2 d) 2 II\J U .. \J \J II Collection of embryoid bodies and further cultivation in suspension (3 d) 5 .. . II • • • •. •. _II I . • Plating of embryoid bodies to 24-well tissue culture plates Il wwwwwwl l • • 5+5 Differentiation of myocytes 5+7 Fusion of myocytes into myotubes - PCR analysis - Immunofluorescence - Patch-clamp studies Fig.
Cell Culture in Pharmaceutical Research by N. E. Fusenig (auth.), N. E. Fusenig, H. Graf (eds.)