By Lalitha Prajna
Read or Download Aravind's atlas of fungal corneal ulcers: clinical features and laboratory identification methods PDF
Similar clinical books
In contemporary a long time, advances in biomedical learn have helped keep or delay the lives of kids around the globe. With stronger cures, baby and adolescent mortality premiums have reduced considerably within the final part century. regardless of those advances, pediatricians and others argue that youngsters haven't shared both with adults in biomedical advances.
Useful magnetic resonance imaging (fMRI) has contributed considerably to growth in neuroscience by means of allowing noninvasive imaging of the "human mind at paintings" lower than physiological stipulations. inside of scientific neuroimaging, fMRI is establishing up a brand new diagnostic box via measuring and visualizing mind functionality.
Comprised solely of scientific instances masking accidents to the proximal interphalangeal (PIP) joint, this concise, useful casebook will supply orthopedic surgeons and hand surgeons with the simplest real-world innovations to correctly deal with the multifaceted surgical concepts for administration of the PIP.
- Cardiac Energetics: Basic Mechanisms and Clinical Implications
- Nutrition: Metabolic and Clinical Applications
- Clinical Hemorheology: Applications in Cardiovascular and Hematological Disease, Diabetes, Surgery and Gynecology
- Mollison's Blood Transfusion in Clinical Medicine, 11th Edition
- Airway Mucus: Basic Mechanisms and Clinical Perspectives
- Clinical Aspects of O2 Transport and Tissue Oxygenation
Extra info for Aravind's atlas of fungal corneal ulcers: clinical features and laboratory identification methods
2 ml 7. Absolute methyl alcohol 8. Distilled water. 1% gold chloride • Rinse with two changes of distilled water • Immerse in 2% sodium thiosulfate solution for 2 minutes • Wash with tap water Staining Procedure for Rapid Identification of Fungi 31 • Counter stain for 1 minute with fresh 1: 5 dilution in distilled water of stock light green solution • Air dry, clean and mount. • Examine by light microscopy using 400X and 1000X magnification Interpretation: Fungal elements stain silver to black, using a low power objective; fungal elements can easily be seen in a tissue section (Fig.
40 Aravind’s Atlas of Fungal Corneal Ulcers TECHNIQUES OF MICROSCOPIC EXAMINATION Principle The simplest technique consists in placing a fragment of the colony between a slide and cover slip. This approach is rapid and often adequate for identification. Delicate fungal structures, however, are often broken by this process, rendering identification difficult. The adhesive tape technique assists in maintaining the integrity of fungal structures by fixing them on the adhesive surface of a piece of transparent (Not frosted) tape.
Examine using a fluorescence microscope for characteristic apple-green (or red if filter is used) chemo fluorescence of fungi and amoebic cysts. We used a Nikon fluorescence microscope with orange-red filter. 20A and B: Blue white fluorescence of fungal hyphae of corneal scraping in KOH Calcofluor staining (A – 200 X magnification, B – 400 X magnification) Interpretation 1. Positive fluorescence is apple-green (or bright orange-red if filter is used) (Fig. 20). 2. Background and other cells stain red-brown.
Aravind's atlas of fungal corneal ulcers: clinical features and laboratory identification methods by Lalitha Prajna