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J. Straathof, S. Panke, and A. Schmid, The production of fine chemicals by biotransformations, Curr. Opin. Biotechnol. 2002, 13, 548–556. M. J. Taschner and D. J. Black, The enzymatic Baeyer–Villiger oxidation: enantioselective synthesis of lactones from mesomeric cyclohexanones, J. Am. Chem. Soc. 1988, 110, 6892–6893. F. H. Westheimer, Coincidences, decarboxylation, and electrostatic effects, Tetrahedron 1995, 51, 3–20. P. Wittung-Stafshede, E. Gomez, A. Ohman, R. Aasa, R. M. Villahermosa, J. Leckner, B.

Kelly and A. L. Zydney, Effects of intermolecular thiol–disulfide interchange reactions on BSA fouling during microfiltration, Biotech. Bioeng. 1994, 44(8), 972−982. G. L. Kenyon, J. A. Gerlt, G. A. Petsko, and J. W. Kozarich, Mandelate racemase: structure–function studies of a pseudosymmetric enzyme, Acc. Chem. Res. 1995, 28, 178–186. H. Kleinkauf and H. von Doehren, A nonribosomal system of peptide biosynthesis, Eur. J. Biochem. 1996, 236, 335–351. J. R. Knowles, Enzyme catalysis: not different, just better, Nature 1991, 350, 121−124.

Cocoa butter* lipase (CRL) fixed-bed, IME Fuji Oil nicotinamide nitrilase 3-stage batch Lonza Guangzhou d-pantothenic acid aldonolactonase Fuji Pharmaceuticals (S)-chloropropionic acid lipase Dow Chemical 6-aminopenillanic acid penicillin amidase fixed-bed, IME 7-aminocephalosporanic acid glutaryl amidase Kundl/Hoechst aspartame® thermolysin soluble enzyme Tosoh/DSM l-aspartate aspartase fixed-bed, IME various d-phenylglycine hydantoinase/ (carbamoylase) resting cells Kanegafuchi d-p-OH-phenylglycine hydantoinase/ carbamoylase resting cells Recordati ampicillin penicillin amidase stirred IME DSM–Gist Brocades l-methionine, l-valine aminoacylase EMR Degussa (Rexim) l-carnitine dehydrase/ hydroxylase whole cells Lonza > 1,000 > 100 various l-dopa β -tyrosinase l-malic acid fumarase fixed-bed, IME Tanabe (S)-methoxyisopropylamine lipase repeated batch BASF (R)-HPOPS hydroxylase batch reactor BASF (R)-mandelic acid nitrilase batch reactor BASF l-alanine l-aspartate-βdecarboxylase IME various Ajinomoto tpy: (metric) tons per year; IME: immobilized enzyme reactor; EMR: enzyme membrane reactor; l-dopa: 3,4-dihydroxyphenylalanine; (R)-HPOPS: (R)-2-(4-hydroxyphenoxy)propionic acid.

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1980s Through 1990s

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