By E. A. Luriya
This translation from Russian into English of the monograph Hematopoi etic and Lymphoid Tissue in tradition via E. A. Luriya offers us for the 1st time with a accomplished evaluation of the large quantity of labor conducted within the USSR during this very important zone of organic study. traditionally, the pioneering tissue tradition experiences of Maksimov, Popov, Chasovnikov, and their colleagues operating in Russia within the early a part of this century supplied a lot of the impetus for next reviews on lymphopoiesis and hematopoiesis in vitro and lots more and plenty of the elemental info used accordingly to accomplish immune responses in vitro and to improve the method for cloning hematopoietic stem cells. really welcome during this translation is the addition of the Appendix, during which Drs. Luriya and Fridenshtein describe in a few aspect the technique that has been constructed of their laboratories for the research of various telephone forms in phone and tissue tradition. for lots of people, this description offers info that had formerly no longer been available to us: porosity of millipore fIlters when it comes to progress houses; colony-forming telephone adhesiveness; professional liferation kinetics of stromal cells; differential effectiveness of assorted media used for tradition of liver cells; information of washing methods for clear out substrates utilized in tradition.
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Additional resources for Hematopoietic and Lymphoid Tissue in Culture
Number of colonies of fibroblasts as a function of number of explanted bone marrow cells. Abscissa: number of explanted cells; ordinate: number of colonies growing. After Fridenshtein et al. (l970b). 24 CHAPTER I 28-60 hr), and cultivation continues later with the addition of nonlabeled thymidine, some of the colony-forming cells will be labeled, while others will not. If each colony is a cell clone, some colonies must thus be entirely labeled and others entirely unlabeled. Mixed colonies consisting oflabeled and unlabeled cells can only be exceptions that depend on the random amalgamation of several colony-forming cells.
Fetal calf serum is known to contain embryo-specific a-globulins. Presumably, it is these a-globulins that have the inhibitory effect on the cultures. As yet, however, no direct evidence is available on the nature of the factor in fetal calf serum that inhibits differentiation and synthetic activity of the hepatic epithelium. On the other hand, these results indicate correlation between the times of maintenance of hematopoiesis in embryonic liver cultures with the duration of synthesis of a-fetoprotein in culture.
A mixture of C14 -labeled amino acids was added to the culture media for 2 or 3 days. After removal of the free label by dialysis, the media were then subjected to electrophoresis. Proteins of the bovine serum contained in the nutrient medium and proteins synthesized by the cultures were observed on the gel after electrophoresis. To obtain the autoradiograph, the polyacrylamide gel was cut into strips, dried, and coated with fluorographic film. After exposure, impressions of the radioactive zone were obtained on the film.
Hematopoietic and Lymphoid Tissue in Culture by E. A. Luriya