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1 Dipotassium Phosphate . . . . . . . . . . . . . . 1 Bacto Brom Thymol Blue . . . . . . . . . . . . 08 Bacto Agar . . . . . . . . . . . . . . . . . . . 1 at 25°C Precautions 1. For Laboratory Use. 2. IRRITANT. IRRITATING TO EYES, RESPIRATORY SYSTEM AND SKIN. Avoid contact with skin and eyes. Do not breathe dust. Wear suitable protective clothing. Keep container tightly closed. FIRST AID: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.

5 Bacto Salicin . . . . . . . . . . . . . . . . . . 5 Triton X-100 . . . . . . . . . . . . . . . . . . . 1 1. 2. 3. 4. Test Procedure 1. 4,5,6 2. 5°C for 3 hours. 3. 2°C and incubate for an additional 21 ± 2 hours. 4. Maintain water level in bath above level of liquid in inoculated tubes. Results5 Gas production in the inverted vial, or dissolved gas that forms fine bubbles when slightly agitated, is a positive reaction indicating the presence of fecal coliforms.

20 Sodium Thioglycollate . . . . . . . . . . . . . . . 2 Sodium Formaldehyde Sulfoxylate . . . . . . . . . . 1 Methylene Blue . . . . . . . . . . . . . . . . 2 at 25°C Prepare Anaerobic Agar per label directions. Inoculate the medium and incubate at 35 ±2°C under anaerobic conditions for 18-48 hours. 3 Precautions 1. For Laboratory Use. 2. Follow proper established laboratory procedures in handling and disposing of infectious material.

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Difco manual on-line by Difco Laboratories


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